Immortalization of Primary Keratinocytes and Its Application to Skin Research
نویسندگان
چکیده
منابع مشابه
Immortalization of Primary Keratinocytes and Its Application to Skin Research
As a major component of the epidermal tissue, a primary keratinocyte has served as an essential tool not only for the study of pathogenesis of skin-related diseases but also for the assessment of potential toxicities of various chemicals used in cosmetics. However, its short lifespan in ex vivo setting has been a great hurdle for many practical applications. Therefore, a number of immortalizati...
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A Method for the Immortalization of Newborn Mouse Skin Keratinocytes
Isolation and culture of mouse primary epidermal keratinocytes is a common technique that allows for easy genetic and environmental manipulation. However, due to their limited lifespan in culture, experiments utilizing primary keratinocytes require large numbers of animals, and are time consuming and expensive. To avoid these issues, we developed a method for the immortalization of primary mous...
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15 صفحه اولDownregulation of 14-3-3σ Prevents Clonal Evolution and Leads to Immortalization of Primary Human Keratinocytes
In human epidermal keratinocytes, replicative senescence, is determined by a progressive decline of clonogenic and dividing cells. Its timing is controlled by clonal evolution, that is, by the continuous transition from stem cells to transient amplifying cells. We now report that downregulation of 14-3-3sigma, which is specifically expressed in human stratified epithelia, prevents keratinocyte ...
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ژورنال
عنوان ژورنال: Biomolecules & Therapeutics
سال: 2015
ISSN: 2005-4483,1976-9148
DOI: 10.4062/biomolther.2015.038